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NSUN2 Polyclonal antibody

Name: NSUN2 Polyclonal antibody
Brand: Proteintech
SKU: 20854-1-AP
Rating: 5.00 (1 reviews)

NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 20854-1-AP

Synonyms

SAKI, RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis of SU-DHL-4 using 20854-1-AP
WB result of NSUN2 antibody (20854-1-AP; 1:40000; room temperature for 1.5 hours) with wild-type and NSUN2 knockout SU-DHL-4 cells.
WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 20854-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20854-1-AP (NSUN2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 20854-1-AP
WB result of NSUN2 antibody (20854-1-AP; 1:60000; room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HepG2 cells.
IP experiment of HeLa using 20854-1-AP
IP result of anti-NSUN2 (IP:20854-1-AP, 4ug; Detection:20854-1-AP 1:1000) with HeLa cells lysate 4000ug.
IHC staining of human breast cancer using 20854-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 20854-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20854-1-AP (NSUN2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver cancer using 20854-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20854-1-AP (NSUN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A549 using 20854-1-AP
Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 20854-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (20854-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HepG2 using 20854-1-AP
Immunofluorescent analysis of HepG2 cells, using NSUN2 antibody 20854-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HeLa using 20854-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human NSUN2 (20854-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Cat No. 20854-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HT-1080 cells, HeLa cells, HepG2 cells, SU-DHL-4 cells, MCF-7 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human breast cancer tissue, human liver cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells, HepG2 cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 29 publications below
WB	See 68 publications below
IHC	See 16 publications below
IF	See 12 publications below
IP	See 5 publications below
CoIP	See 1 publications below

Product Information

20854-1-AP targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種
Full Name	NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight	767 aa, 86 kDa
Observed molecular weight	90-100 kDa
GenBank accession number	BC001041
Gene symbol	NSUN2
Gene ID (NCBI)	54888
RRID	AB_10693629
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 20854-1-AP	Download protocol
IHC protocol for NSUN2 antibody 20854-1-AP	Download protocol
IF protocol for NSUN2 antibody 20854-1-AP	Download protocol
IP protocol for NSUN2 antibody 20854-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Signal Transduct Target Ther tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis Authors - Sunyang Ying View Article
human	WB	Nat Biotechnol Identification of direct targets and modified bases of RNA cytosine methyltransferases. Authors - Khoddami Vahid V View Article
mouse	WB	Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. Authors - Deniz Simsek View Article
human	WB,IHC	Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. Authors - Xin Chen View Article
human	WB	Nat Protoc Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. Authors - Vahid Khoddami View Article
human	WB	Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach Authors - Liang Lu View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Sarah (Verified Customer) (01-05-2021)

Works perfectly for WB. Tested on HeLa cells and HEK293T.

Applications: Western Blot
Primary Antibody Dilution: 1/1000
Cell Tissue Type: HeLa and HEL293T cells

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSUN2 Polyclonal antibody

NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis of SU-DHL-4 using 20854-1-AP

WB analysis using 20854-1-AP

WB analysis using 20854-1-AP

WB analysis using 20854-1-AP

WB analysis of HepG2 using 20854-1-AP

IP experiment of HeLa using 20854-1-AP

IHC staining of human breast cancer using 20854-1-AP

IHC staining of human testis using 20854-1-AP

IHC staining of human liver cancer using 20854-1-AP

IF Staining of A549 using 20854-1-AP

IF Staining of HepG2 using 20854-1-AP

IF Staining of HepG2 using 20854-1-AP

FC experiment of HeLa using 20854-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis

Identification of direct targets and modified bases of RNA cytosine methyltransferases.

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.

Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP.

Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach

Reviews

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