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NSUN2 Polyclonal antibody
NSUN2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 20854-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HT-1080 cells, HeLa cells, HepG2 cells, MCF-7 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human liver cancer tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells, HepG2 cells |
Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 29 publications below |
WB | See 65 publications below |
IHC | See 16 publications below |
IF | See 11 publications below |
IP | See 5 publications below |
CoIP | See 1 publications below |
Product Information
20854-1-AP targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種 |
Full Name | NOL1/NOP2/Sun domain family, member 2 |
Calculated molecular weight | 767 aa, 86 kDa |
Observed molecular weight | 90-100 kDa |
GenBank accession number | BC001041 |
Gene symbol | NSUN2 |
Gene ID (NCBI) | 54888 |
RRID | AB_10693629 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NSUN2 antibody 20854-1-AP | Download protocol |
IHC protocol for NSUN2 antibody 20854-1-AP | Download protocol |
IF protocol for NSUN2 antibody 20854-1-AP | Download protocol |
IP protocol for NSUN2 antibody 20854-1-AP | Download protocol |
FC protocol for NSUN2 antibody 20854-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther tRF-Gln-CTG-026 ameliorates liver injury by alleviating global protein synthesis | ||
Nat Biotechnol Identification of direct targets and modified bases of RNA cytosine methyltransferases. | ||
Cell The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity. | ||
Nat Cell Biol 5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs. | ||
Nat Protoc Transcriptome-wide target profiling of RNA cytosine methyltransferases using the mechanism-based enrichment procedure Aza-IP. | ||
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Sarah (Verified Customer) (01-05-2021) | Works perfectly for WB. Tested on HeLa cells and HEK293T.
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