• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

NSUN2 Monoclonal antibody, PBS Only

NSUN2 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H5A2

Cat no : 66580-1-PBS

Synonyms

hTrm4, MISU, NSUN2, SAKI, TRM4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of COLO 320 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of COLO 320 using 66580-1-Ig (same clone as 66580-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66580-1-Ig (NSUN2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    WB analysis of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    WB result of NSUN2 antibody (66580-1-Ig; 1:40000; room temperature for 1.5 hours) with negative control and NSUN2 overexpressed HepG2 cells. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IP experiment of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    IP result of anti-NSUN2 (IP:66580-1-Ig, 4ug; Detection:66580-1-Ig 1:4000) with HepG2 cells lysate 1920 ug. This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human oesophagus cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NSUN2 Monoclonal antibody (66580-1-Ig)

    IHC staining of human breast cancer using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66580-1-Ig (NSUN2 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Monoclonal antibody (66580-1-Ig)

    IF Staining of HepG2 using 66580-1-Ig (same clone as 66580-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66580-1-Ig (NSUN2 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66580-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66580-1-PBS targets NSUN2 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.