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  • Featured Product
  • KD/KO Validated

NSUN2 Recombinant antibody

NSUN2 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230186G2

Cat no : 82894-2-RR

Synonyms

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NSUN2 Recombinant antibody (82894-2-RR)

    WB analysis of HeLa using 82894-2-RR

    WB result of NSUN2 antibody (82894-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN2 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using NSUN2 Recombinant antibody (82894-2-RR)

    WB analysis using 82894-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82894-2-RR (NSUN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NSUN2 Recombinant antibody (82894-2-RR)

    IF Staining of HeLa using 82894-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Recombinant antibody (82894-2-RR)

    IF Staining of HepG2 using 82894-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-2-RR, Clone: 230186G2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using NSUN2 Recombinant antibody (82894-2-RR)

    FC experiment of HeLa using 82894-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-2-RR, Clone:230186G2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 82894-2-RR

    Affinity and Kinetic Characterization of 82894-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82894-2-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, HT-1080 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82894-2-RR targets NSUN2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.

Protocols

Product Specific Protocols
WB protocol for NSUN2 antibody 82894-2-RRDownload protocol
IF protocol for NSUN2 antibody 82894-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols