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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSUN2 Recombinant antibody, PBS Only

NSUN2 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230186H8

Cat no : 82894-7-PBS

Synonyms

RNA cytosine C(5)-methyltransferase NSUN2, Myc-induced SUN domain-containing protein, mRNA cytosine C(5)-methyltransferase, EC:2.1.1.-, EC:2.1.1.203, EC:2.1.1.-

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSUN2 Recombinant antibody (82894-7-RR)

    WB analysis using 82894-7-RR (same clone as 82894-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82894-7-RR (NSUN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using NSUN2 Recombinant antibody (82894-7-RR)

    IHC staining of human stomach cancer using 82894-7-RR (same clone as 82894-7-PBS)

    Immunohistochemical analysis of paraffin-embedded stomach cancer slide using 82894-7-RR (NSUN2 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN2 Recombinant antibody (82894-7-RR)

    IF Staining of HepG2 using 82894-7-RR (same clone as 82894-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN2 antibody (82894-7-RR, Clone: 230186H8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).CL594-Phalloidin (red). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using NSUN2 Recombinant antibody (82894-7-RR)

    FC experiment of HeLa using 82894-7-RR (same clone as 82894-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug NSUN2 Recombinant antibody (82894-7-RR, Clone:230186H8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82894-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82894-7-RR

    Affinity and Kinetic Characterization of 82894-7-RR

    Biolayer interferometry (BLl) kinetic assays of 82894-7-RR against Human NSUN2 were performed. The affinity constant is below 1 pM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82894-7-PBS targets NSUN2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN2 fusion protein Ag14791 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 2
Calculated molecular weight 767 aa, 86 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberBC001041
Gene symbol NSUN2
Gene ID (NCBI) 54888
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NSUN2, also known as SAKI or Misu (Myc-induced SUN-domain-containing protein), is a methyltransferase which catalyses (cytosine-5-)-methylation of tRNA. NSUN2 is direct target gene of c-Myc and may act downstream of Myc to regulate epidermal cell growth and proliferation. NSUN2 is overexpressed in various cancer tissues and may be a valuable target for cancer therapy and a cancer diagnostic marker. Recently a splicing mutation in NSUN2 has been identified as the cause of a Dubowitz-like syndrome, an autosomal recessive disorder.