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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSUN4 Polyclonal antibody (29786-1-AP)

    WB analysis using 29786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29786-1-AP (NSUN4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NSUN4 Polyclonal antibody (29786-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 29786-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 29786-1-AP (NSUN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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NSUN4 Polyclonal antibody

NSUN4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29786-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, SH-SY5Y cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29786-1-AP targets NSUN4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN4 fusion protein Ag31821 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 4
Calculated molecular weight 384 aa, 43 kDa
Observed molecular weight40 kDa
GenBank accession numberBC014441
Gene Symbol NSUN4
Gene ID (NCBI) 387338
RRIDAB_2935480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96CB9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly. The calculated MW of NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.(PMID:22949673, 24516400)

Protocols

Product Specific Protocols
WB protocol for NSUN4 antibody 29786-1-APDownload protocol
IHC protocol for NSUN4 antibody 29786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols