NSUN4 Recombinant antibody
NSUN4 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
241115B7
Cat no : 84275-1-RR
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HT-29 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
The immunogen of 84275-1-RR is NSUN4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NSUN4 fusion protein Ag31828 相同性解析による交差性が予測される生物種 |
| Full Name | NOL1/NOP2/Sun domain family, member 4 |
| Calculated molecular weight | 384 aa, 43 kDa |
| Observed molecular weight | ~43 kDa |
| GenBank accession number | BC014441 |
| Gene symbol | NSUN4 |
| Gene ID (NCBI) | 387338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN4 antibody 84275-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
