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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN4 Recombinant antibody (84275-3-RR)

    IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NSUN4 Recombinant antibody (84275-3-RR)

    IF Staining of HepG2 using 84275-3-RR (same clone as 84275-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NSUN4 antibody (84275-3-RR, Clone: 241115G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84275-3-PBS in a different storage buffer formulation.

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NSUN4 Recombinant antibody, PBS Only

NSUN4 Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA
Cat No. 84275-3-PBS
Clone No.241115G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Synonyms

RP4 603I14.2, 5-methylcytosine tRNA methyltransferase NSUN4, 5-methylcytosine rRNA methyltransferase NSUN4, 241115G11

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84275-3-PBS targets NSUN4 in IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN4 fusion protein Ag31828 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 4
Calculated molecular weight 384 aa, 43 kDa
GenBank accession numberBC014441
Gene Symbol NSUN4
Gene ID (NCBI) 387338
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96CB9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NSUN4 belongs to a family of RNA 5-methylcytosine (m5C) methyltransferases (MTases). MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts with MTERF4 to facilitate mitoribosomal assembly.