- Featured Product
- KD/KO Validated
NSUN5 Polyclonal antibody
NSUN5 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, RIP, ELISA
Conjugate
Unconjugated
Cat no : 15449-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, human kidney tissue, human placenta tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 10 publications below |
| IHC | See 3 publications below |
| IF | See 3 publications below |
| RIP | See 1 publications below |
Product Information
15449-1-AP targets NSUN5 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSUN5 fusion protein Ag7711 相同性解析による交差性が予測される生物種 |
| Full Name | NOL1/NOP2/Sun domain family, member 5 |
| Calculated molecular weight | 46.6 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC008084 |
| Gene symbol | NSUN5 |
| Gene ID (NCBI) | 55695 |
| RRID | AB_2153808 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN5 antibody 15449-1-AP | Download protocol |
| IHC protocol for NSUN5 antibody 15449-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach | ||
Nat Chem Biol Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.
| ||
Glia Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells. | ||
Cells Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.
| ||
Front Oncol Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma. | ||
Mol Brain Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.
|
