Featured Product
KD/KO Validated

NSUN5 Polyclonal antibody

Name: NSUN5 Polyclonal antibody
Brand: Proteintech
SKU: 15449-1-AP

NSUN5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15449-1-AP

Synonyms

NOL1, NOL1 related protein, NOL1R, NSUN5, NSUN5A, p120, WBSCR20, WBSCR20A, Ynl022cL

Validation Data Gallery

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AllWBIHC

WB analysis of HeLa using 15449-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15449-1-AP
WB result of NSUN5 antibody (15449-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN5 transfected HeLa cells.
WB analysis of A549 using 15449-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 15449-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15449-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin cancer using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 15449-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, human kidney tissue, human placenta tissue
Positive IHC detected in	mouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 10 publications below
IHC	See 3 publications below
IF	See 3 publications below
RIP	See 1 publications below

Product Information

15449-1-AP targets NSUN5 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NSUN5 fusion protein Ag7711 相同性解析による交差性が予測される生物種
Full Name	NOL1/NOP2/Sun domain family, member 5
Calculated molecular weight	46.6 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC008084
Gene symbol	NSUN5
Gene ID (NCBI)	55695
RRID	AB_2153808
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NSUN5 antibody 15449-1-AP	Download protocol
IHC protocol for NSUN5 antibody 15449-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach Authors - Liang Lu View Article
human	WB	Nat Chem Biol Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Authors - Wei Dai KD Validated View Article
mouse	WB,IF	Glia Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells. Authors - Tingting Zhang View Article
mouse	IF	Cells Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase. Authors - Zihao Yuan KO Validated View Article
human	IHC	Front Oncol Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma. Authors - Zhenyuan Han View Article
mouse	WB,IF	Mol Brain Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex. Authors - Peipei Chen KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSUN5 Polyclonal antibody

NSUN5 Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis of HeLa using 15449-1-AP

WB analysis of HeLa using 15449-1-AP

WB analysis of A549 using 15449-1-AP

WB analysis of human kidney using 15449-1-AP

WB analysis of human placenta using 15449-1-AP

IHC staining of mouse skeletal muscle using 15449-1-AP

IHC staining of mouse skeletal muscle using 15449-1-AP

IHC staining of human skin cancer using 15449-1-AP

IHC staining of human skin cancer using 15449-1-AP

IHC staining of human placenta using 15449-1-AP

IHC staining of human placenta using 15449-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

Cognitive deficits in mice lacking Nsun5, a cytosine-5 RNA methyltransferase, with impairment of oligodendrocyte precursor cells.

Agenesis and Hypomyelination of Corpus Callosum in Mice Lacking Nsun5, an RNA Methyltransferase.

Identification of Expression Patterns and Potential Prognostic Significance of m5C-Related Regulators in Head and Neck Squamous Cell Carcinoma.

Expression of the RNA methyltransferase Nsun5 is essential for developing cerebral cortex.