Validation Data Gallery

Filter: AllWBIHC

WB analysis of HeLa using 15449-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15449-1-AP
WB result of NSUN5 antibody (15449-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSUN5 transfected HeLa cells.
WB analysis of human kidney using 15449-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 15449-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 15449-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 15449-1-AP (NSUN5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse skeletal muscle using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin cancer using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer using 15449-1-AP (NSUN5 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 15449-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15449-1-AP (NSUN5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

NSUN5 Polyclonal antibody

NSUN5 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15449-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA

Synonyms

28S rRNA (cytosine-C(5))-methyltransferase, EC:2.1.1.-, NOL1, NOL1R, NOL1-related protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, A549 cells, human kidney tissue, human placenta tissue
Positive IHC detected in	mouse skeletal muscle tissue, human placenta tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 12 publications below
IHC	See 5 publications below
IF	See 5 publications below
IP	See 1 publications below
RIP	See 2 publications below

Product Information

15449-1-AP targets NSUN5 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7711 Product name: Recombinant human NSUN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 117-466 aa of BC008084 Sequence: RNEDLLEVGSRPGPASQLPRFVRVNTLKTCSDDVVDYFKRQGFSYQGRASSLDDLRALKGKHFLLDPLMPELLVFPAQTDLHEHPLYRAGHLILQDRASCLPAMLLDPPPGSHVIDACAAPGNKTSHLAALLKNQGKIFAFDLDAKRLASMATLLARAGVSCCELAEEDFLAVSPSDPRYHEVHYILLDPSCSGSGMPSRQLEEPGAGTPSPVRLHALAGFQQRALCHALTFPSLQRLVYSTCSLCQEENEDVVRDALQQNPGAFRLAPALPAWPHRGLSTFPGAEHCLRASPETTLSSGFFVAVIERVEVPSSASQAKASAPERTPSPAPKRKKRQQRAAAGACTPPCT 相同性解析による交差性が予測される生物種
Full Name	NOL1/NOP2/Sun domain family, member 5
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC008084
Gene Symbol	NSUN5
Gene ID (NCBI)	55695
RRID	AB_2153808
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96P11
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.

Protocols

Product Specific Protocols
IHC protocol for NSUN5 antibody 15449-1-AP	Download protocol
WB protocol for NSUN5 antibody 15449-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun Epitranscriptomic subtyping, visualization, and denoising by global motif visualization Authors - Jianheng Liu View Article
human	WB	Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach Authors - Liang Lu View Article
human	WB	Nat Chem Biol Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. Authors - Wei Dai KD Validated View Article
human	WB	Cell Death Dis m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization Authors - Ning Wang View Article
human	RIP,IHC	Phytomedicine Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure Authors - Liqiao Huang View Article
rat	WB	Cell Death Discov The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells. Authors - Jie Liu KD Validated View Article

Validation Data Gallery

WB analysis of HeLa using 15449-1-AP

WB analysis of HeLa using 15449-1-AP

WB analysis of human kidney using 15449-1-AP

WB analysis of human placenta using 15449-1-AP

WB analysis of A549 using 15449-1-AP

IHC staining of mouse skeletal muscle using 15449-1-AP

IHC staining of mouse skeletal muscle using 15449-1-AP

IHC staining of human skin cancer using 15449-1-AP

IHC staining of human skin cancer using 15449-1-AP

IHC staining of human placenta using 15449-1-AP

IHC staining of human placenta using 15449-1-AP

NSUN5 Polyclonal antibody

NSUN5 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Epitranscriptomic subtyping, visualization, and denoising by global motif visualization

Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach

Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation.

m5C-dependent cross-regulation between nuclear reader ALYREF and writer NSUN2 promotes urothelial bladder cancer malignancy through facilitating RABL6/TK1 mRNAs splicing and stabilization

Niujiao Dihuang Jiedu decoction promotes SLC7A11 m5C methylation modification against ferroptosis in acute-on-chronic liver failure

The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells.

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