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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NSUN5 Recombinant antibody (84768-2-RR)

    WB analysis using 84768-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84768-2-RR (NSUN5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NSUN5 Recombinant antibody (84768-2-RR)

    IF Staining of HeLa using 84768-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NSUN5 Recombinant antibody (84768-2-RR)

    IF Staining of A431 using 84768-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NSUN5 antibody (84768-2-RR, Clone: 242168D5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84768-2-RR

    Affinity and Kinetic Characterization of 84768-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84768-2-RR against Human NSUN5 were performed. The affinity constant is 0.69 nM.

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NSUN5 Recombinant antibody

NSUN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84768-2-RR
Clone No.242168D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

NOL1-related protein, NOL1R, NOL1, EC:2.1.1.-, 28S rRNA (cytosine-C(5))-methyltransferase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa cells, A549 cells
Positive IF/ICC detected inHeLa cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84768-2-RR targets NSUN5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NSUN5 fusion protein Ag7711 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 5
Calculated molecular weight 47 kDa
Observed molecular weight47~51 kDa
GenBank accession numberBC008084
Gene Symbol NSUN5
Gene ID (NCBI) 55695
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96P11
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN5 is a conserved RNA methyltransferase belonging to the Nop2/SUN domain family, which is highly conserved from archaea to eukaryotes and has close contact with RNA methylation because of their S-adenosyl methionine binding-domain. NSUN5 was a promoter in the progression of CRC mainly through cell cycle regulation (PMID: 32774740). Loss of NSUN5 induces growth phenotypes in mice and different mammalian cells, which is likely connected to the requirement of NSUN5 for ribosomal RNA modification and normal global protein translation, especially in highly proliferative cells (PMID: 31722427). Western blot analysis detected NSUN5 at an apparent molecular mass of 47 kDa.

Protocols

Product Specific Protocols
WB protocol for NSUN5 antibody 84768-2-RRDownload protocol
IF protocol for NSUN5 antibody 84768-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols