- Featured Product
- KD/KO Validated
NSUN6 Polyclonal antibody
NSUN6 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, RIP, ELISA
Conjugate
Unconjugated
Cat no : 17240-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Neuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 10 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
| RIP | See 1 publications below |
Product Information
17240-1-AP targets NSUN6 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NSUN6 fusion protein Ag11084 相同性解析による交差性が予測される生物種 |
| Full Name | NOL1/NOP2/Sun domain family, member 6 |
| Calculated molecular weight | 469 aa, 52 kDa |
| Observed molecular weight | 52-55 kDa |
| GenBank accession number | BC035778 |
| Gene symbol | NSUN6 |
| Gene ID (NCBI) | 221078 |
| RRID | AB_2878367 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| IHC protocol for NSUN6 antibody 17240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Base-resolution m5C profiling across the mammalian transcriptome by bisulfite-free enzyme-assisted chemical labeling approach | ||
Nucleic Acids Res Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6.
| ||
Mol Syst Biol CIGAR-seq, a CRISPR/Cas-based method for unbiased screening of novel mRNA modification regulators.
| ||
Nat Struct Mol Biol Genome-wide identification of mRNA 5-methylcytosine in mammals.
| ||
Exp Ther Med Loss of NSUN6 inhibits osteosarcoma progression by downregulating EEF1A2 expression and activation of Akt/mTOR signaling pathway via m5C methylation
|
