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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSUN6 Monoclonal antibody

NSUN6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H1H8

Cat no : 68614-1-Ig

Synonyms

4933414E04Rik, FLJ23743, NOPD1, NSUN6

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NSUN6 Monoclonal antibody (68614-1-Ig)

    WB analysis using 68614-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68614-1-Ig (NSUN6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, HEK-293 cells, HepG2 cells, K-562 cells, Mo7e cells, Ramos cells, Raji cells, pig liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68614-1-Ig targets NSUN6 in WB, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NSUN6 fusion protein Ag11084 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 6
Calculated molecular weight 469 aa, 52 kDa
Observed molecular weight50-52 kDa
GenBank accession numberBC035778
Gene symbol NSUN6
Gene ID (NCBI) 221078
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with the Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis.

Protocols

Product Specific Protocols
WB protocol for NSUN6 antibody 68614-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols