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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NSUN7 Polyclonal antibody

NSUN7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17546-1-AP

Synonyms

NSUN7

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using NSUN7 Polyclonal antibody (17546-1-AP)

    WB analysis using 17546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NSUN7 Polyclonal antibody (17546-1-AP)

    WB analysis using 17546-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17546-1-AP (NSUN7 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat testis tissue using NSUN7 Polyclonal antibody (17546-1-AP)

    IHC staining of rat testis using 17546-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 17546-1-AP (NSUN7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inmouse liver tissue, mouse testis tissue, rat liver tissue
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17546-1-AP targets NSUN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NSUN7 fusion protein Ag11650 相同性解析による交差性が予測される生物種
Full Name NOL1/NOP2/Sun domain family, member 7
Calculated molecular weight 475 aa, 54 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC036568
Gene symbol NSUN7
Gene ID (NCBI) 79730
RRIDAB_2878415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NSUN7 antibody 17546-1-APDownload protocol
IHC protocol for NSUN7 antibody 17546-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Oncol

Comprehensive Analysis of 5-Methylcytosine (m5C) Regulators and the Immune Microenvironment in Pancreatic Adenocarcinoma to Aid Immunotherapy.

Authors - Ronglin Wang
View Article