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Tested Applications
| Positive WB detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84795-1-RR targets NSUN7 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | NSUN7 fusion protein Ag11650 相同性解析による交差性が予測される生物種 |
| Full Name | NOL1/NOP2/Sun domain family, member 7 |
| Calculated molecular weight | 718 aa, 81 kDa |
| Observed molecular weight | 81~90 kDa |
| GenBank accession number | BC036568 |
| Gene Symbol | NSUN7 |
| Gene ID (NCBI) | 79730 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NE18 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NSUN7 (NOP2/Sun RNA methyltransferase family member 7) is involved in mitochondrial rRNA processing in post-meiotic sperm and is predicted to act upstream of or within flagellated sperm motility and sperm mitochondrion organization. In humans, Nsun7 encodes a protein with 718-amino acid and 3697-bp belonging to the methyltransferase superfamily (PMID: 37173708). The failure to produce NSUN7 would lead to the impairment in activity and motility of sperm flagella, leading to sperm motility deficit and infertility (PMID: 25702163).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NSUN7 antibody 84795-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |