Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, human placenta tissue, A549 cells, NCl-H1299 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue, NCI-H1299 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
15223-1-AP targets NT5C2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NT5C2 fusion protein Ag7438 相同性解析による交差性が予測される生物種 |
| Full Name | 5'-nucleotidase, cytosolic II |
| Calculated molecular weight | 65 kDa |
| Observed molecular weight | 60-65 kDa |
| GenBank accession number | BC001595 |
| Gene Symbol | NT5C2 |
| Gene ID (NCBI) | 22978 |
| RRID | AB_2153946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49902 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NT5C2 antibody 15223-1-AP | Download protocol |
| IF protocol for NT5C2 antibody 15223-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
BMC Med Genomics Identification of differential gene expression profile from peripheral blood cells of military pilots with hypertension by RNA sequencing analysis. | ||
Cell Death Dis Inosine enhances tumor mitochondrial respiration by inducing Rag GTPases and nascent protein synthesis under nutrient starvation |