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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NT5C3 Polyclonal antibody

NT5C3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11393-1-AP

Synonyms

5' nucleotidase, cytosolic III, cN III, Cytosolic 5 nucleotidase 3, Cytosolic 5 nucleotidase III, NT5C3, p36, P5N 1, P5'N 1, P5N1, PN I, PSN1, Pyrimidine 5 nucleotidase 1, UMPH, UMPH1

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    WB analysis of human skeletal muscle using 11393-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse placenta tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    WB analysis of mouse placenta using 11393-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    WB analysis of human skeletal muscle using 11393-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    WB analysis of mouse kidney using 11393-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11393-1-AP (NT5C3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    IP experiment of mouse kidney using 11393-1-AP

    IP result of anti-NT5C3 (IP:11393-1-AP, 4ug; Detection:11393-1-AP 1:500) with mouse kidney tissue lysate 3000 ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NT5C3 Polyclonal antibody (11393-1-AP)

    IHC staining of human colon cancer using 11393-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11393-1-AP (NT5C3 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

Positive WB detected inhuman skeletal muscle tissue, mouse kidney tissue, mouse placenta tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11393-1-AP targets NT5C3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5C3 fusion protein Ag1949 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, cytosolic III
Calculated molecular weight 297 aa, 34 kDa
Observed molecular weight 33-38 kDa
GenBank accession numberBC015856
Gene symbol NT5C3
Gene ID (NCBI) 51251
RRIDAB_2267361
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5C3 antibody 11393-1-APDownload protocol
IHC protocol for NT5C3 antibody 11393-1-APDownload protocol
IP protocol for NT5C3 antibody 11393-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Biotechnol

Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution

Authors - Qing Dai
View Article