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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NT5C3L Polyclonal antibody

NT5C3L Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 16292-1-AP

Synonyms

NT5C3B, NT5C3L

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse stomach tissue using NT5C3L Polyclonal antibody (16292-1-AP)

    WB analysis of mouse stomach using 16292-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using NT5C3L Polyclonal antibody (16292-1-AP)

    WB analysis of human testis using 16292-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using NT5C3L Polyclonal antibody (16292-1-AP)

    WB analysis of human testis using 16292-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 16292-1-AP (NT5C3L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using NT5C3L Polyclonal antibody (16292-1-AP)

    IP experiment of mouse testis using 16292-1-AP

    IP result of anti-NT5C3L (IP:16292-1-AP, 4ug; Detection:16292-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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Tested Applications

Positive WB detected inmouse stomach tissue, human testis tissue, mouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16292-1-AP targets NT5C3L in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5C3L fusion protein Ag9458 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, cytosolic III-like
Calculated molecular weight 292 aa, 34 kDa
Observed molecular weight 30-34 kDa
GenBank accession numberBC014132
Gene symbol NT5C3L
Gene ID (NCBI) 115024
RRIDAB_2251490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5C3L antibody 16292-1-APDownload protocol
IP protocol for NT5C3L antibody 16292-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols