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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NT5DC1 Polyclonal antibody

NT5DC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24102-1-AP

Synonyms

NT5C2L1, EC:3.1.3.-, 5'-nucleotidase domain-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using NT5DC1 Polyclonal antibody (24102-1-AP)

    WB analysis of MCF-7 using 24102-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NT5DC1 Polyclonal antibody (24102-1-AP)

    WB analysis of Jurkat using 24102-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NT5DC1 Polyclonal antibody (24102-1-AP)

    WB analysis of HepG2 using 24102-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24102-1-AP (NT5DC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NT5DC1 Polyclonal antibody (24102-1-AP)

    IHC staining of human lung cancer using 24102-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NT5DC1 Polyclonal antibody (24102-1-AP)

    IHC staining of human lung cancer using 24102-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using NT5DC1 Polyclonal antibody (24102-1-AP)

    IHC staining of human thyroid cancer using 24102-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using NT5DC1 Polyclonal antibody (24102-1-AP)

    IHC staining of human thyroid cancer using 24102-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 24102-1-AP (NT5DC1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NT5DC1 Polyclonal antibody (24102-1-AP)

    IF Staining of HepG2 using 24102-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24102-1-AP (NT5DC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24102-1-AP targets NT5DC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5DC1 fusion protein Ag20916 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase domain containing 1
Calculated molecular weight 455 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC015138
Gene symbol NT5DC1
Gene ID (NCBI) 221294
RRIDAB_2879431
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5DC1 antibody 24102-1-APDownload protocol
IHC protocol for NT5DC1 antibody 24102-1-APDownload protocol
IF protocol for NT5DC1 antibody 24102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols