• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

CD73 Polyclonal antibody

CD73 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 12231-1-AP

Synonyms

NT5E, 5'-nucleotidase, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73)

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CD73 Polyclonal antibody (12231-1-AP)

    WB analysis using 12231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A375 cells using CD73 Polyclonal antibody (12231-1-AP)

    WB analysis of A375 using 12231-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using CD73 Polyclonal antibody (12231-1-AP)

    WB analysis of human spleen using 12231-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CD73 Polyclonal antibody (12231-1-AP)

    WB analysis of K-562 using 12231-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CD73 Polyclonal antibody (12231-1-AP)

    WB analysis of mouse brain using 12231-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12231-1-AP (CD73 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human lung cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human lung cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human colon cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human stomach cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human colon cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human stomach cancer using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of mouse kidney using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of mouse kidney using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human stomach using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human stomach using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using CD73 Polyclonal antibody (12231-1-AP)

    IHC staining of human stomach using 12231-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12231-1-AP (CD73 antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CD73 Polyclonal antibody (12231-1-AP)

    IF Staining of mouse small intestine using 12231-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse small intestine tissue using CD73 Polyclonal antibody (12231-1-AP)

    IF Staining of mouse small intestine using 12231-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using CD73 antibody (12231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inA375 cells, human spleen tissue, K-562 cells, mouse brain tissue, HT-29 cells, HuH-7 cells, Jurkat cells
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue, human stomach tissue, mouse kidney tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse small intestine tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12231-1-AP targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD73 fusion protein Ag2869 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
Observed molecular weight 63-70 kDa
GenBank accession numberBC015940
Gene symbol CD73
Gene ID (NCBI) 4907
RRIDAB_2154081
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries (CALJA).

Protocols

Product Specific Protocols
WB protocol for CD73 antibody 12231-1-APDownload protocol
IHC protocol for CD73 antibody 12231-1-APDownload protocol
IF protocol for CD73 antibody 12231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,WB,IF

Int J Biol Sci

CD73/NT5E-mediated ubiquitination of AURKA regulates alcohol-related liver fibrosis via modulating hepatic stellate cell senescence

Authors - Zhenni Liu
View Article
humanWB,IHC

Br J Cancer

Norepinephrine inhibits CD8+ T-cell infiltration and function, inducing anti-PD-1 mAb resistance in lung adenocarcinoma

Authors - Qishun Geng
View Article
mouseIF

Theranostics

GATA4-driven miR-206-3p signatures control orofacial bone development by regulating osteogenic and osteoclastic activity.

Authors - Shuyu Guo
View Article
humanIF

Cell Mol Biol Lett

Reciprocal negative feedback between Prrx1 and miR-140-3p regulates rapid chondrogenesis in the regenerating antler

Authors - Pengfei Hu
View Article
IHC

Theranostics

A sericin/ graphene oxide composite scaffold as a biomimetic extracellular matrix for structural and functional repair of calvarial bone.

Authors - Chao Qi
View Article
humanWB

Biochim Biophys Acta Mol Basis Dis

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Authors - Ling Zhou
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alessandro (Verified Customer) (12-09-2023)

good product for ICC

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: ROs
FH

Neta (Verified Customer) (07-28-2022)

staining did not work

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: mouse small intestine, fixed frozen