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  • Featured Product
  • KD/KO Validated

CD73 Monoclonal antibody, PBS Only

CD73 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G5G8

Cat no : 67789-1-PBS

Synonyms

NT5E, 5'-NT, 5'-deoxynucleotidase, 5' nucleotidase, ecto (CD73), 5 nucleotidase

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using CD73 Monoclonal antibody (67789-1-Ig)

    WB analysis of HepG2 using 67789-1-Ig (same clone as 67789-1-PBS)

    WB result of NT5E/CD73 antibody (67789-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NT5E,CD73 transfected HepG2 cells. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CD73 Monoclonal antibody (67789-1-Ig)

    WB analysis using 67789-1-Ig (same clone as 67789-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of rat kidney using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of rat kidney using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 67789-1-Ig (NT5E/CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of mouse kidney using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IHC staining of mouse kidney using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 67789-1-Ig (NT5E,CD73 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human appendicitis tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of human appendicitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human appendicitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of rat liver using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat liver tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of rat liver using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat liver tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CD73 Monoclonal antibody (67789-1-Ig)

    IF Staining of human tonsillitis using 67789-1-Ig (same clone as 67789-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using NT5E/CD73 antibody (67789-1-Ig, Clone: 1G5G8 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67789-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67789-1-PBS targets CD73 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD73 fusion protein Ag30616 相同性解析による交差性が予測される生物種
Full Name 5'-nucleotidase, ecto (CD73)
Calculated molecular weight 29 kDa, 63 kDa
Observed molecular weight60-70 kDa
GenBank accession numberBC015940
Gene symbol CD73
Gene ID (NCBI) 4907
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CD73, also known as ecto-5'-nucleotidase (5'-NT), is a 70-kDa, glycosyl-phosphatidylinositol-linked membrane-bound glycoprotein found in most tissues (PMID: 18404475; 20179192). CD73 is an ectoenzyme that catalyzes the dephosphorylation of AMP and other nucleoside monophosphates (PMID: 9553767). In the human immune system, CD73 is expressed on subsets of T and B cells, on germinal center follicular dendritic cells, and on thymic medullary reticular fibroblasts and epithelial cells (PMID: 2137649; 9553767). CD73 is highly expressed in many human solid tumors and is closely involved in cancer progression (PMID: 20179192).