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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using NT5M Polyclonal antibody (20765-1-AP)

    WB analysis of mouse brain using 20765-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NT5M Polyclonal antibody (20765-1-AP)

    WB analysis of mouse skeletal muscle using 20765-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using NT5M Polyclonal antibody (20765-1-AP)

    IP experiment of mouse skeletal muscle using 20765-1-AP

    IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

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NT5M Polyclonal antibody

NT5M Polyclonal Antibody for WB, IP, ELISA
Cat No. 20765-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Synonyms

dNT-2, DNT2, dNT 2, Deoxy-5'-nucleotidase 2, 5',3'-nucleotidase, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20765-1-AP targets NT5M in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5M fusion protein Ag14743 相同性解析による交差性が予測される生物種
Full Name 5',3'-nucleotidase, mitochondrial
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC035838
Gene Symbol NT5M
Gene ID (NCBI) 56953
RRIDAB_10733338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NPB1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5M antibody 20765-1-APDownload protocol
IP protocol for NT5M antibody 20765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols