• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NT5M Polyclonal antibody

NT5M Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 20765-1-AP

Synonyms

dNT-2, DNT2, dNT 2, Deoxy-5'-nucleotidase 2, 5',3'-nucleotidase, mitochondrial

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using NT5M Polyclonal antibody (20765-1-AP)

    WB analysis of mouse brain using 20765-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using NT5M Polyclonal antibody (20765-1-AP)

    WB analysis of mouse skeletal muscle using 20765-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20765-1-AP (NT5M antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse skeletal muscle tissue using NT5M Polyclonal antibody (20765-1-AP)

    IP experiment of mouse skeletal muscle using 20765-1-AP

    IP result of anti-NT5M (IP:20765-1-AP, 4ug; Detection:20765-1-AP 1:600) with mouse skeletal muscle tissue lysate 2200ug.

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20765-1-AP targets NT5M in WB, IP, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NT5M fusion protein Ag14743 相同性解析による交差性が予測される生物種
Full Name 5',3'-nucleotidase, mitochondrial
Calculated molecular weight 228 aa, 26 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC035838
Gene symbol NT5M
Gene ID (NCBI) 56953
RRIDAB_10733338
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NT5M antibody 20765-1-APDownload protocol
IP protocol for NT5M antibody 20765-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols