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Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using NTB-A/SLAMF6 Polyclonal antibody (22711-1-AP)

    WB analysis of Daudi using 22711-1-AP (same clone as 22711-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 22711-1-AP (SLAMF6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NTB-A/SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human colon cancer using 22711-1-AP (same clone as 22711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using NTB-A/SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human colon cancer using 22711-1-AP (same clone as 22711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22711-1-AP (SLAMF6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NTB-A/SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human tonsillitis using 22711-1-AP (same clone as 22711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using NTB-A/SLAMF6 Polyclonal antibody (22711-1-AP)

    IHC staining of human tonsillitis using 22711-1-AP (same clone as 22711-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22711-1-AP (SLAMF6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22711-1-PBS in a different storage buffer formulation.

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NTB-A/SLAMF6 Polyclonal antibody, PBS Only

NTB-A/SLAMF6 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22711-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22711-1-PBS targets NTB-A/SLAMF6 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NTB-A/SLAMF6 fusion protein Ag18517 相同性解析による交差性が予測される生物種
Full Name SLAM family member 6
Calculated molecular weight 332 aa, 37 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC114495
Gene Symbol SLAMF6
Gene ID (NCBI) 114836
RRIDAB_2879154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96DU3
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Signaling lymphocyte activation molecule 6 (SLAMF6) (Ly108 in mice, NTB-A or SF2000 in humans) is a homophilic receptor belonging to the superfamily immunoglobulin (Ig) domain-containing molecules (PMID:31199820). SLAMF6 is a type I transmembrane protein with two extracellular immunoglobins (Ig)-like domains and three cytoplasmic tyrosine-based signaling motifs, one of which is immunoreceptor tyrosine-based switch motif (PMID:31315913). SLAMF6 is expressed on a wide variety of immune cells including T cells (also TFH), B cells, NK cells (expressed in humans only), double positive thymocytes, eosinophils, and neutrophils (mouse only) (PMID:11862385).