Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, Y79 cells, HeLa cells, K-562 cells |
| Positive IP detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
14918-1-AP targets NTHL1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NTHL1 fusion protein Ag6734 相同性解析による交差性が予測される生物種 |
| Full Name | nth endonuclease III-like 1 (E. coli) |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000391 |
| Gene Symbol | NTHL1 |
| Gene ID (NCBI) | 4913 |
| RRID | AB_2154555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78549 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NTHL1, also named as Endonuclease III-like protein 1 or OCTS3, is a 312 amino acid protein, which belongs to the Nth/MutY family. NTHL1 localizes in the nucleus and is widely expressed with highest levels in heart and lowest levels in lung and liver. NTHL1 Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity and incises damaged DNA at cytosines, thymines and guanines. NTHL1 acts on a damaged strand, 5' from the damaged site and is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NTHL1 antibody 14918-1-AP | Download protocol |
| IP protocol for NTHL1 antibody 14918-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Mitochondrial respiratory function is preserved under cysteine starvation via glutathione catabolism in NSCLC |