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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using TrkA Recombinant antibody (83477-7-RR)

    WB analysis of K-562 using 83477-7-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83477-7-RR (TrkA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using TrkA Recombinant antibody (83477-7-RR)

    WB analysis of SH-SY5Y using 83477-7-RR

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 83477-7-RR (TrkA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83477-7-RR

    Affinity and Kinetic Characterization of 83477-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83477-7-RR against Human NTRK1 were performed. The affinity constant is below 1 pM.

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TrkA Recombinant antibody

TrkA Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83477-7-RR
Clone No.240440D12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

NTRK1, Trk A, TRK, p140 TrkA, MTC

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inK-562 cells, SH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83477-7-RR targets TrkA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TrkA fusion protein Ag32759 相同性解析による交差性が予測される生物種
Full Name neurotrophic tyrosine kinase, receptor, type 1
Calculated molecular weight88 kDa
Observed molecular weight140 kDa
GenBank accession numberNM_001007792
Gene Symbol NTRK1
Gene ID (NCBI) 4914
RRIDAB_3671106
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP04629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NTRK1(Neurotrophic tyrosine kinase receptor 1) is located on chromosome 1q23.1 and is responsible for a rare genetic disorder, CIPA (congenital insensitivity to pain with anhidrosis) (PMID: 37907480). As a neurotrophin receptor, NTRK1 is abundantly expressed in human neuronal tissues and plays an integral role in the development and function of the human nervous system (PMID: 27843590). NTRK1 also regulates the survival and proliferation of cholinergic neurons (PMID: 20054812).

Protocols

Product Specific Protocols
WB protocol for TrkA antibody 83477-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols