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  • KD/KO Validated

ARK5 Polyclonal antibody

ARK5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22723-1-AP

Synonyms

AMPK related protein kinase 5, ARK5, KIAA0537, NUAK family SNF1 like kinase 1, NUAK1


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Tested Applications

Positive WB detected inmouse brain tissue, rat heart tissue, mouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22723-1-AP targets ARK5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARK5 fusion protein Ag18612 相同性解析による交差性が予測される生物種
Full Name NUAK family, SNF1-like kinase, 1
Calculated molecular weight 661 aa, 74 kDa
Observed molecular weight 74 kDa
GenBank accession numberBC152462
Gene symbol ARK5
Gene ID (NCBI) 9891
RRIDAB_2879157
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUAK1 is a member of the human adenosine monophosphate (AMP)-activated protein kinase family that has been identified as a key tumor cell survival factor. It is also named as ARK5, KIAA0537, OMPHK1 and belongs to the protein kinase superfamily. The physiological role of NUAK1 is potentially to suppress glucose uptake through negative regulation of INS signaling in oxidative muscle. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for ARK5 antibody 22723-1-APDownload protocol
IHC protocol for ARK5 antibody 22723-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS Genet

A Mouse Model Uncovers LKB1 as an UVB-Induced DNA Damage Sensor Mediating CDKN1A (p21WAF1/CIP1) Degradation.

Authors - Rosaura Esteve-Puig
  • KD Validated
humanIHC

Front Immunol

Identification of senescence-related subtypes, establishment of a prognosis model, and characterization of a tumor microenvironment infiltration in breast cancer

Authors - Yanling Zhou
  • KD Validated
humanWB

Biomed Pharmacother

Discovery of a novel NUAK1 inhibitor against pancreatic cancer.

Authors - Myeong-Seong Seo
humanIHC

Int J Biol Sci

miR-424-5p represses the metastasis and invasion of intrahepatic cholangiocarcinoma by targeting ARK5.

Authors - Jingbang Wu
mouseIF

Sci Rep

High-throughput kinase inhibitor screening reveals roles for Aurora and Nuak kinases in neurite initiation and dendritic branching.

Authors - Sara M Blazejewski
humanWB

Int J Immunopathol Pharmacol

Cancer susceptibility 18 positively regulates NUAK Family Kinase 1 expression to promote migration and invasion via sponging of miR-5586-5p in cervical cancer cells

Authors - Jingrong Wang