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KD/KO Validated

NUAK2 Polyclonal antibody

Name: NUAK2 Polyclonal antibody
Brand: Proteintech
SKU: 11592-1-AP

NUAK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11592-1-AP

Synonyms

NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase

Validation Data Gallery

Filter:

AllWBIHCIF

WB analysis of Y79 using 11592-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11592-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11592-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 11592-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 11592-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 11592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11592-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human renal cell carcinoma using 11592-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human renal cell carcinoma using 11592-1-AP
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11592-1-AP (NUAK2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH3T3 cells using 11592-1-AP
NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) .

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Cat No. 11592-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Y79 cells, HeLa cells
Positive IHC detected in	human kidney tissue, human renal cell carcinoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below
IHC	See 1 publications below
IF	See 2 publications below

Product Information

11592-1-AP targets NUAK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUAK2 fusion protein Ag2031 相同性解析による交差性が予測される生物種
Full Name	NUAK family, SNF1-like kinase, 2
Calculated molecular weight	628 aa, 70 kDa
Observed molecular weight	70 kDa
GenBank accession number	BC017306
Gene symbol	NUAK2
Gene ID (NCBI)	81788
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306).

Protocols

Product Specific Protocols
WB protocol for NUAK2 antibody 11592-1-AP	Download protocol
IHC protocol for NUAK2 antibody 11592-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma. Authors - Namiki Takeshi T View Article
human	WB, IF	Cancer Res NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation. Authors - Takeshi Namiki KD Validated View Article
human	WB	Diabetologia Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes. Authors - Rune A A KD Validated View Article
human	WB, IF	Ann Clin Biochem Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients. Authors - Doaa Hussein Zineldeen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUAK2 Polyclonal antibody

NUAK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Validation Data Gallery

WB analysis of Y79 using 11592-1-AP

WB analysis of HeLa using 11592-1-AP

WB analysis of HeLa using 11592-1-AP

WB analysis of Y79 using 11592-1-AP

WB analysis of Y79 using 11592-1-AP

IHC staining of human kidney using 11592-1-AP

IHC staining of human kidney using 11592-1-AP

IHC staining of human renal cell carcinoma using 11592-1-AP

IHC staining of human renal cell carcinoma using 11592-1-AP

IF Staining of NIH3T3 cells using 11592-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma.

NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation.

Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes.

Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients.