Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, Raji cells |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
14343-1-AP targets NUB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUB1 fusion protein Ag5668 相同性解析による交差性が予測される生物種 |
| Full Name | negative regulator of ubiquitin-like proteins 1 |
| Calculated molecular weight | 71 kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | BC046354 |
| Gene Symbol | NUB1 |
| Gene ID (NCBI) | 51667 |
| RRID | AB_2155889 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5A7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Proteasome-associated protein NEDD8 ultimate buster 1 (NUB1) is an interferon-inducible protein that down-regulates NEDD8 expression and its conjugation system. Over-expression of NUB1 induces a growth-inhibitory effect in cells, and it mediates anti-proliferative actions and apoptosis in renal cell carcinoma cells through cell-cycle regulation. Expression of NUB1 leads to decreased modification of p53 with NEDD8 and stimulation of p53 ubiquitination. Recent finding revealed that NUB1, by regulating GSK3β levels, modulates tau phosphorylation and aggregation, and is a key player in neurodegeneration associated with tau pathology.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUB1 antibody 14343-1-AP | Download protocol |
| IF protocol for NUB1 antibody 14343-1-AP | Download protocol |
| IP protocol for NUB1 antibody 14343-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun The role of NUB1 in α-synuclein degradation in Lewy body disease model mice. | ||
Neuropathology Ubiquitin-negative, eosinophilic neuronal cytoplasmic inclusions associated with stress granules and autophagy: an immunohistochemical investigation of two cases. | ||
Brain Pathol Phosphorylated NUB1 distinguishes α-synuclein in Lewy bodies from that in glial cytoplasmic inclusions in multiple system atrophy |