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  • Featured Product
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NUBP1 Polyclonal antibody

NUBP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 18011-1-AP

Synonyms

Nucleotide binding protein 1, Nbp35, NBP1, NBP 1, NBP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUBP1 Polyclonal antibody (18011-1-AP)

    WB analysis using 18011-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18011-1-AP (NUBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUBP1 Polyclonal antibody (18011-1-AP)

    WB analysis of HeLa using 18011-1-AP

    WB result of NUBP1 antibody (18011-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUBP1 transfected HeLa cells.

  • Immunoprecipitation (IP) experiment of Jurkat cells using NUBP1 Polyclonal antibody (18011-1-AP)

    IP experiment of Jurkat using 18011-1-AP

    IP result of anti-NUBP1 (IP:18011-1-AP, 4ug; Detection:18011-1-AP 1:300) with Jurkat cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using NUBP1 Polyclonal antibody (18011-1-AP)

    IHC staining of human stomach using 18011-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18011-1-AP (NUBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, LNCaP cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18011-1-AP targets NUBP1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUBP1 fusion protein Ag12510 相同性解析による交差性が予測される生物種
Full Name nucleotide binding protein 1 (MinD homolog, E. coli)
Calculated molecular weight 309 aa, 33 kDa
Observed molecular weight36-40 kDa
GenBank accession numberBC100290
Gene symbol NUBP1
Gene ID (NCBI) 4682
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUBP1 antibody 18011-1-APDownload protocol
IHC protocol for NUBP1 antibody 18011-1-APDownload protocol
IP protocol for NUBP1 antibody 18011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Sci Adv

Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer

Authors - Subhadip Mukhopadhyay
View Article