Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 15409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 15409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.
WB analysis using 15409-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 15409-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15409-1-AP (NUBP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 15409-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 15409-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human pancreas using 15409-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human pancreas using 15409-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 15409-1-AP (NUBP2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of L02 using 15409-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed L02 cells using NUBP2 antibody (15409-1-AP) at dilution of 1:200 and Cy3-conjugated Goat Anti-Rabbit IgG(H+L).

NUBP2 Polyclonal antibody

NUBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15409-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Nucleotide-binding protein 2, Nucleotide binding protein 2, NUBP1, NBP 2, Cytosolic Fe-S cluster assembly factor NUBP2

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, mouse pancreas tissue, L02 cells, U-251 cells, HepG2 cells, NIH/3T3 cells
Positive IHC detected in	human skeletal muscle tissue, human pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:5000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below

Product Information

15409-1-AP targets NUBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUBP2 fusion protein Ag7676 相同性解析による交差性が予測される生物種
Full Name	nucleotide binding protein 2 (MinD homolog, E. coli)
Calculated molecular weight	29 kDa
Observed molecular weight	30-33 kDa
GenBank accession number	BC002768
Gene Symbol	NUBP2
Gene ID (NCBI)	10101
RRID	AB_10858630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5Y2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for NUBP2 antibody 15409-1-AP	Download protocol
IHC protocol for NUBP2 antibody 15409-1-AP	Download protocol
IF protocol for NUBP2 antibody 15409-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Struct Mol Biol Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators. Authors - Nima N Naseri View Article
human	WB	J Biol Chem Iron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery. Authors - Xiaorui Fan View Article
human,mouse	WB,IHC	Mol Cell Biochem Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer Authors - Danfeng Lan View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15409-1-AP

WB analysis using 15409-1-AP

WB analysis using 15409-1-AP

WB analysis of mouse pancreas using 15409-1-AP

IHC staining of human skeletal muscle using 15409-1-AP

IHC staining of human skeletal muscle using 15409-1-AP

IHC staining of human pancreas using 15409-1-AP

IHC staining of human pancreas using 15409-1-AP

IF Staining of L02 using 15409-1-AP

NUBP2 Polyclonal antibody

NUBP2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Aggregation of mutant cysteine string protein-α via Fe-S cluster binding is mitigated by iron chelators.

Iron-Regulated Assembly of the Cytosolic Iron-Sulfur Cluster Biogenesis Machinery.

Abnormal upregulation of NUBP2 contributes to cancer progression in colorectal cancer