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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Nucleobindin 1 Polyclonal antibody

Nucleobindin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10228-1-AP

Synonyms

NUCB1, Nucleobindin-1, NUC, CALNUC

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    WB analysis of MCF-7 using 10228-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    WB analysis of A431 using 10228-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    WB analysis of HeLa using 10228-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    WB analysis of HepG2 using 10228-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    IP experiment of HepG2 using 10228-1-AP

    IP result of anti-nucleobindin 1 (IP:10228-1-AP, 4ug; Detection:10228-1-AP 1:1000) with HepG2 cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human brain tissue using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    IHC staining of human brain using 10228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using nucleobindin 1 Polyclonal antibody (10228-1-AP)

    IHC staining of human brain using 10228-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Nucleobindin 1 Polyclonal antibody (10228-1-AP)

    IF Staining of mouse brain using 10228-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using nucleobindin 1 antibody (10228-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Nucleobindin 1 Polyclonal antibody (10228-1-AP)

    IF Staining of HepG2 using 10228-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 10228-1-AP (nucleobindin 1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using Nucleobindin 1 Polyclonal antibody (10228-1-AP)

    FC experiment of HepG2 using 10228-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug nucleobindin 1 Polyclonal antibody (10228-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inMCF7 cells, A431 cells, HeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10228-1-AP targets Nucleobindin 1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Nucleobindin 1 fusion protein Ag0290 相同性解析による交差性が予測される生物種
Full Name nucleobindin 1
Calculated molecular weight 55 kDa
Observed molecular weight 63 kDa
GenBank accession numberBC002356
Gene symbol NUCB1
Gene ID (NCBI) 4924
RRIDAB_2153655
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nucleobindin-1 (NUCB1), also known as Calnuc, is a highly conserved, multifunctional protein widely expressed in tissues and cells.It has been proposed to contain both calcium- and DNA-binding motifs, including two EF-hands, a basic amino-acid-rich region and a leucine zipper.Western blotting revealed a band at approximately 63 kDa, corresponding to the expected size of the NUCB1 protein(PMID: 15255074)

Protocols

Product Specific Protocols
WB protocol for Nucleobindin 1 antibody 10228-1-APDownload protocol
IHC protocol for Nucleobindin 1 antibody 10228-1-APDownload protocol
IF protocol for Nucleobindin 1 antibody 10228-1-APDownload protocol
IP protocol for Nucleobindin 1 antibody 10228-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
MouseWB

Int Orthop

Proteomic analysis of mineralising osteoblasts identifies novel genes related to bone matrix mineralisation.

Authors - Saad Fawzy A FA
View Article