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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUCB2/nesfatin-1 Recombinant antibody, PBS Only (Detector)

NUCB2/nesfatin-1 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241037D8

Cat no : 83917-2-PBS

Synonyms

NUCB2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4, DNA binding protein NEFA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    WB analysis using 83917-2-RR (same clone as 83917-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    WB analysis using 83917-2-RR (same clone as 83917-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83917-2-RR (NUCB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    FC experiment of A431 using 83917-2-RR (same clone as 83917-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR)

    FC experiment of HepG2 using 83917-2-RR (same clone as 83917-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUCB2/nesfatin-1 Recombinant antibody (83917-2-RR, Clone:241037D8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83917-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00864-3

    Cytometric bead array standard curve of MP00864-3

    Cytometric bead array standard curve of MP00864-3, NUCB2/nesfatin-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83917-1-PBS. Detection antibody: 83917-2-PBS. Standard: Ag25152. Range: 1.25-40 ng/mL.

  • Affinity and Kinetic Characterization of 83917-2-RR

    Affinity and Kinetic Characterization of 83917-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83917-2-RR against Human NUCB2/nesfatin-1 were performed. The affinity constant is 8.99 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83917-2-PBS targets NUCB2/nesfatin-1 as part of a matched antibody pair:

MP00864-3: 83917-1-PBS capture and 83917-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag25152 相同性解析による交差性が予測される生物種
Full Name nucleobindin 2
Calculated molecular weight 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberNM_005013
Gene symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).