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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUCB2/nesfatin-1 Polyclonal antibody, PBS Only

NUCB2/nesfatin-1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30604-1-PBS

Synonyms

NUCB2, Nucleobindin-2, nucleobindin 2, NEFA, Gastric cancer antigen Zg4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    WB analysis using 30604-1-AP (same clone as 30604-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    WB analysis using 30604-1-AP (same clone as 30604-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP (same clone as 30604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30604-1-AP (same clone as 30604-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30604-1-AP (NUCB2/nesfatin-1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using NUCB2/nesfatin-1 Polyclonal antibody (30604-1-AP)

    IF Staining of U2OS using 30604-1-AP (same clone as 30604-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NUCB2/nesfatin-1 antibody (30604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30604-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30604-1-PBS targets NUCB2/nesfatin-1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUCB2/nesfatin-1 fusion protein Ag30704 相同性解析による交差性が予測される生物種
Full Name nucleobindin 2
Calculated molecular weight 50 kDa
Observed molecular weight46-50 kDa
GenBank accession numberNM_005013
Gene symbol NUCB2
Gene ID (NCBI) 4925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NucB2, an anorexigenic molecule, is expressed mainly in the hypothalamus, particularly in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN). NucB2 is also expressed in the subfornical organ (SFO). Because the SON and PVN are involved in body fluid regulation, NucB2 may be involved in dehydration-induced anorexia. NUCB2 is composed of a signal peptide of 24 amino acids in the N-terminal region and a protin structure containing 396 amino acids. Nesfatin-1 is an 82-amino-acid peptide hormone cleaved from nucleobindin2 (NUCB2).