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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUDCD2 Polyclonal antibody

NUDCD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 21205-1-AP

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDCD2 Polyclonal antibody (21205-1-AP)

    WB analysis using 21205-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    WB analysis of human brain using 21205-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    WB analysis of HEK-293 using 21205-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    WB analysis of HepG2 using 21205-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human kidney tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    WB analysis of human kidney using 21205-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 21205-1-AP (NUDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IP experiment of HepG2 using 21205-1-AP

    IP result of anti-NUDCD2 (IP:21205-1-AP, 4ug; Detection:21205-1-AP 1:500) with HepG2 cells lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    IHC staining of human kidney using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    IHC staining of human kidney using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    IHC staining of human kidney using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21205-1-AP (NUDCD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    IHC staining of human breast cancer using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUDCD2 Polyclonal antibody (21205-1-AP)

    IHC staining of human breast cancer using 21205-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21205-1-AP (NUDCD2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IF Staining of HepG2 using 21205-1-AP

    Immunofluorescent analysis of HepG2 cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IF Staining of HepG2 using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IF Staining of HeLa using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IF Staining of HepG2 using 21205-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (21205-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDCD2 Polyclonal antibody (21205-1-AP)

    IF Staining of HeLa using 21205-1-AP

    Immunofluorescent analysis of Hela cells, using NUDCD2 antibody 21205-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293 cells, human brain tissue, human kidney tissue, mouse brain tissue, rat brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21205-1-AP targets NUDCD2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDCD2 fusion protein Ag15579 相同性解析による交差性が予測される生物種
Full Name NudC domain containing 2
Calculated molecular weight 157 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC017934
Gene symbol NUDCD2
Gene ID (NCBI) 134492
RRIDAB_10732725
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).

Protocols

Product Specific Protocols
WB protocol for NUDCD2 antibody 21205-1-APDownload protocol
IHC protocol for NUDCD2 antibody 21205-1-APDownload protocol
IF protocol for NUDCD2 antibody 21205-1-APDownload protocol
IP protocol for NUDCD2 antibody 21205-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Mol Life Sci

RetSat stabilizes mitotic chromosome segregation in pluripotent stem cells

Authors - Wanzhi Cai
View Article