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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDCD2 Recombinant antibody (83217-5-RR)

    WB analysis using 83217-5-RR (same clone as 83217-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83217-5-RR (NUDCD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    WB analysis of HEK-293 using 83217-5-RR (same clone as 83217-5-PBS)

    WB result of NUDCD2 antibody (83217-5-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDCD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    IP experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    IP result of anti-NUDCD2 (IP:83217-5-RR, 4ug; Detection:83217-5-RR 1:4000) with HepG2 cells lysate 1085 ug. This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    IF Staining of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUDCD2 antibody (83217-5-RR, Clone: 230513A5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using NUDCD2 Recombinant antibody (83217-5-RR)

    FC experiment of HepG2 using 83217-5-RR (same clone as 83217-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug NUDCD2 Recombinant antibody (83217-5-RR, Clone:230513A5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83217-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83217-5-RR

    Affinity and Kinetic Characterization of 83217-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83217-5-RR against Human NUDCD2 were performed. The affinity constant is below 1 pM.

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  • KD/KO Validated

NUDCD2 Recombinant antibody, PBS Only

NUDCD2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 83217-5-PBS
Clone No.230513A5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

NudCL2, NudC domain-containing protein 2, NudC domain containing 2, 230513A5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83217-5-PBS targets NUDCD2 in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NUDCD2 fusion protein Ag15579 相同性解析による交差性が予測される生物種
Full Name NudC domain containing 2
Calculated molecular weight 157 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC017934
Gene Symbol NUDCD2
Gene ID (NCBI) 134492
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8WVJ2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

NUDCD2, also known as NudCL2, is an HSP90 cochaperone that regulates sister chromatid cohesion during mitosis. NUDCD2 also plays a role in ciliogenesis(PMID: 30368549, 34480124). NUDCD2 regulates the LIS1/dynein pathway by stabilizing LIS1 with Hsp90 chaperone(PMID: 20133715).