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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT3/4/10/11 Polyclonal antibody (17508-1-AP)

    WB analysis using 17508-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using NUDT3/4/10/11 Polyclonal antibody (17508-1-AP)

    WB analysis of mouse brain using 17508-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUDT3/4/10/11 Polyclonal antibody (17508-1-AP)

    WB analysis of human brain using 17508-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using NUDT3/4/10/11 Polyclonal antibody (17508-1-AP)

    WB analysis of human brain using 17508-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17508-1-AP (NUDT10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT3/4/10/11 Polyclonal antibody (17508-1-AP)

    IF Staining of HeLa using 17508-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using NUDT3/4/10/11 antibody (17508-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17508-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

NUDT10, DIPP3A, Diphosphoinositol polyphosphate phosphohydrolase 3-alpha, Diadenosine hexaphosphate hydrolase, Diadenosine 5',5'''-P1,P6-hexaphosphate hydrolase 3-alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17508-1-AP targets NUDT3/4/10/11 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT3/4/10/11 fusion protein Ag11711 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 10
Calculated molecular weight 164 aa, 19 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC050700
Gene Symbol NUDT10
Gene ID (NCBI) 170685
RRIDAB_2154145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

Protocols

Product Specific Protocols
WB protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
IF protocol for NUDT3/4/10/11 antibody 17508-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols