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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUDT10 Monoclonal antibody

NUDT10 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3A8D5

Cat no : 68478-1-Ig

Synonyms

APS2, DIPP 3 alpha, DIPP3 alpha, DIPP3a, hAps2, hDIPP3alpha, Nudix motif 10, NUDT10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT10 Monoclonal antibody (68478-1-Ig)

    WB analysis using 68478-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68478-1-Ig (NUDT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUDT10 Monoclonal antibody (68478-1-Ig)

    WB analysis using 68478-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68478-1-Ig (NUDT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inU2OS cells, rat brain tissue, HEK-293 cells, rat testis tissue, mouse testis tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68478-1-Ig targets NUDT10 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NUDT10 fusion protein Ag11878 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 10
Calculated molecular weight 164 aa, 19 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC050700
Gene symbol NUDT10
Gene ID (NCBI) 170685
RRIDAB_3085189
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT10 (nucleoside diphosphate linked moiety X-type motif 10), also named as DIPP3A or APS2, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228).

Protocols

Product Specific Protocols
WB protocol for NUDT10 antibody 68478-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols