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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT12 Polyclonal antibody (17487-1-AP)

    WB analysis using 17487-1-AP

    mouse liver tissues were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using NUDT12 Polyclonal antibody (17487-1-AP)

    WB analysis of HeLa using 17487-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17487-1-AP (NUDT12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT12 Polyclonal antibody (17487-1-AP)

    IF Staining of HeLa using 17487-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 17487-1-AP (NUDT12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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NUDT12 Polyclonal antibody

NUDT12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 17487-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

Nucleoside diphosphate-linked moiety X motif 12, NADH pyrophosphatase NUDT12, NAD-capped RNA hydrolase NUDT12, EC:3.6.1.22, EC:3.6.1.-

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17487-1-AP targets NUDT12 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT12 fusion protein Ag11557 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 12
Calculated molecular weight 462 aa, 52 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC041099
Gene Symbol NUDT12
Gene ID (NCBI) 83594
RRIDAB_2236164
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQG2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUDT12 antibody 17487-1-APDownload protocol
IF protocol for NUDT12 antibody 17487-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Dis

A tumor-specific modulation of heterogeneous ribonucleoprotein A0 promotes excessive mitosis and growth in colorectal cancer cells.

Authors - Hiroaki Konishi
View Article