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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    WB result of NUDT21 antibody (66335-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUDT21 transfected NIH/3T3 cells. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    WB analysis of NIH/3T3 using 66335-1-Ig (same clone as 66335-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    WB analysis of HEK-293 using 66335-1-Ig (same clone as 66335-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    WB analysis of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    WB analysis of MCF-7 using 66335-1-Ig (same clone as 66335-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66335-1-Ig (NUDT21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of mouse brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of mouse liver using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using NUDT21 Monoclonal antibody (66335-1-Ig)

    IHC staining of human brain using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66335-1-Ig (NUDT21 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    IF Staining of HeLa using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUDT21 antibody (66335-1-Ig, Clone: 1C4C8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:400 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUDT21 Monoclonal antibody (66335-1-Ig)

    IF Staining of HepG2 using 66335-1-Ig (same clone as 66335-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66335-1-Ig(NUDT21 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66335-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

NUDT21 Monoclonal antibody, PBS Only

NUDT21 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66335-1-PBS
Clone No.1C4C8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Nucleoside diphosphate-linked moiety X motif 21, CPSF5, CPSF25, Cleavage factor Im complex 25 kDa subunit, CFIm25

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66335-1-PBS targets NUDT21 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUDT21 fusion protein Ag12260 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 21
Calculated molecular weight 26 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC001403
Gene Symbol NUDT21
Gene ID (NCBI) 11051
RRIDAB_2881715
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43809
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cleavage and polyadenylation specific factor 5, 25 kDa (CPSF5, synonym: CFIM25) is one subunit of a cleavage factor required for 3' RNA cleavage and polyadenylation processing. The interaction of CPSF5 with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors.