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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT22 Monoclonal antibody (67921-1-Ig)

    WB analysis using 67921-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67921-1-Ig (NUDT22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using NUDT22 Monoclonal antibody (67921-1-Ig)

    WB analysis of LNCaP using 67921-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67921-1-Ig (NUDT22 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using NUDT22 Monoclonal antibody (67921-1-Ig)

    FC experiment of Jurkat using 67921-1-Ig

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NUDT22 (67921-1-Ig, Clone:3C3H9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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NUDT22 Monoclonal antibody

NUDT22 Monoclonal Antibody for WB, ELISA, FC (Intra)
Cat No. 67921-1-Ig
Clone No.3C3H9

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, ELISA, FC (Intra)

Synonyms

UDPG pyrophosphatase, PP11246, NUDT 22, Nucleoside diphosphate-linked moiety X motif 22, EC:3.6.1.45

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, LNCaP cells, K-562 cells, HSC-T6 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67921-1-Ig targets NUDT22 in WB, ELISA, FC (Intra) applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NUDT22 fusion protein Ag14787 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 22
Calculated molecular weight 303 aa, 33 kDa
Observed molecular weight33 kDa
GenBank accession numberBC006129
Gene Symbol NUDT22
Gene ID (NCBI) 84304
RRIDAB_2918673
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BRQ3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human NUDT22 belongs to the diverse NUDIX family of proteins, is a Mg2+-dependent UDP-glucose and UDP-galactose hydrolase, producing UMP and glucose 1-phosphate or galactose 1-phosphate.

Protocols

Product Specific Protocols
WB protocol for NUDT22 antibody 67921-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols