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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUDT3/4/10/11 Polyclonal antibody (20542-1-AP)

    WB analysis using 20542-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20542-1-AP (NUDT3/4/10/11 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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NUDT3/4/10/11 Polyclonal antibody

NUDT3/4/10/11 Polyclonal Antibody for WB, ELISA
Cat No. 20542-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

DIPP, DIPP 1, DIPP1, Nudix motif 3, NUDT3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20542-1-AP targets NUDT3/4/10/11 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUDT3/4/10/11 fusion protein Ag14587 相同性解析による交差性が予測される生物種
Full Name nudix (nucleoside diphosphate linked moiety X)-type motif 3
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 20-30 kDa
GenBank accession numberBC007727
Gene Symbol NUDT3
Gene ID (NCBI) 11165
RRIDAB_10696178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95989
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NUDT3 (nucleoside diphosphate linked moiety X-type motif 3), also named as DIPP1, belongs to the subgroup of phosphohydrolases that preferentially attack diphosphoinositol polyphosphates (PMID: 12105228, 12689335). Five human isoforms of DIPP have been found and named as DIPP type 1(NUDT3), type 2α/β(NUDT4), type 3α(NUDT10) and type 3β(NUDT11), which have the similar molecular weight and high homology (PMID: 12105228). This antibody can recognize all the 5 isoforms of DIPP (NUDT3/4/10/11).

Protocols

Product Specific Protocols
WB protocol for NUDT3/4/10/11 antibody 20542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols