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KD/KO Validated

NUDT6 Polyclonal antibody

Name: NUDT6 Polyclonal antibody
Brand: Proteintech
SKU: 11181-1-AP

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11181-1-AP

Synonyms

gfg, FGF2AS, FGF AS, FGF 2, EC:3.6.1.-

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of Jurkat using 11181-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11181-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 11181-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11181-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 11181-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 11181-1-AP (NUDT6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 11181-1-AP
IP result of anti-NUDT6 (IP:11181-1-AP, 4ug; Detection:11181-1-AP 1:500) with HepG2 cells lysate 4000ug.
IHC staining of human liver using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 11181-1-AP
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11181-1-AP (NUDT6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11181-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using NUDT6 antibody (11181-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Cat No. 11181-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HepG2 cells, human heart tissue, rat heart tissue, rat liver tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, mouse brain tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11181-1-AP targets NUDT6 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUDT6 fusion protein Ag1638 相同性解析による交差性が予測される生物種
Full Name	nudix (nucleoside diphosphate linked moiety X)-type motif 6
Calculated molecular weight	36 kDa
Observed molecular weight	72 kDa
GenBank accession number	BC009842
Gene symbol	NUDT6
Gene ID (NCBI)	11162
RRID	AB_2236190
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

NUDT6, also named as FGF2AS and GFG, belongs to the Nudix hydrolase family. It may contribute to the regulation of cell proliferation. NUDT6 maybe a candidate gene for fat deposition. The 72 kDa is a homodimer form of NUDT6.

Protocols

Product Specific Protocols
WB protocol for NUDT6 antibody 11181-1-AP	Download protocol
IHC protocol for NUDT6 antibody 11181-1-AP	Download protocol
IF protocol for NUDT6 antibody 11181-1-AP	Download protocol
IP protocol for NUDT6 antibody 11181-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat	WB,IF	J Physiol FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling Authors - Burak Uzay View Article
human	WB	Mol Endocrinol Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2. Authors - MacFarlane Leigh-Ann LA View Article
human	WB	Mol Carcinog Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression. Authors - MacFarlane Leigh-Ann LA View Article
mouse	WB	Cancer Sci PMID: 29532562 Authors - Ji-Chang Wang View Article
rat	WB	Genes (Basel) Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA. Authors - Mark Baguma-Nibasheka KD Validated View Article
human	WB	Wound Repair Regen GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis. Authors - Xinying Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUDT6 Polyclonal antibody

NUDT6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 11181-1-AP

WB analysis of HepG2 using 11181-1-AP

WB analysis of rat heart using 11181-1-AP

WB analysis of human heart using 11181-1-AP

WB analysis of rat liver using 11181-1-AP

IP experiment of HepG2 using 11181-1-AP

IHC staining of human liver using 11181-1-AP

IHC staining of human liver using 11181-1-AP

IHC staining of mouse brain using 11181-1-AP

IHC staining of mouse brain using 11181-1-AP

IHC staining of rat colon using 11181-1-AP

IHC staining of rat colon using 11181-1-AP

IF Staining of HepG2 using 11181-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FGF2 gene's antisense protein, NUDT6, plays a depressogenic role by promoting inflammation and suppressing neurogenesis without altering FGF2 signalling

Regulation of fibroblast growth factor-2 by an endogenous antisense RNA and by argonaute-2.

Regulation of FGF-2 by an endogenous antisense RNA: effects on cell adhesion and cell-cycle progression.

PMID: 29532562

Regulation of fibroblast growth factor-2 expression and cell cycle progression by an endogenous antisense RNA.

GHK-Cu-liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis.