• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of A375 using 12515-1-AP (same clone as 12515-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    IP experiment of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    IP result of anti-NUFIP1 (IP:12515-1-AP, 4ug; Detection:12515-1-AP 1:800) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUFIP1 Polyclonal antibody (12515-1-AP)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUFIP1 Polyclonal antibody (12515-1-AP)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    IF Staining of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUFIP1 antibody (12515-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

  • Featured Product
  • KD/KO Validated

NUFIP1 Polyclonal antibody, PBS Only

NUFIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12515-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12515-1-PBS targets NUFIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUFIP1 fusion protein Ag3197 相同性解析による交差性が予測される生物種
Full Name nuclear fragile X mental retardation protein interacting protein 1
Calculated molecular weight 56 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC017745
Gene Symbol NUFIP1
Gene ID (NCBI) 26747
RRIDAB_2298759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHK0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].