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  • Featured Product
  • KD/KO Validated

NUFIP1 Polyclonal antibody, PBS Only

NUFIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12515-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A375 cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    WB analysis of A375 using 12515-1-AP (same clone as 12515-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12515-1-AP (NUFIP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    IP experiment of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    IP result of anti-NUFIP1 (IP:12515-1-AP, 4ug; Detection:12515-1-AP 1:800) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUFIP1 Polyclonal antibody (12515-1-AP)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using NUFIP1 Polyclonal antibody (12515-1-AP)

    IHC staining of human ovary tumor using 12515-1-AP (same clone as 12515-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12515-1-AP (NUFIP1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using NUFIP1 Polyclonal antibody (12515-1-AP)

    IF Staining of HeLa using 12515-1-AP (same clone as 12515-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NUFIP1 antibody (12515-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12515-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12515-1-PBS targets NUFIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUFIP1 fusion protein Ag3197 相同性解析による交差性が予測される生物種
Full Name nuclear fragile X mental retardation protein interacting protein 1
Calculated molecular weight 56 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC017745
Gene symbol NUFIP1
Gene ID (NCBI) 26747
RRIDAB_2298759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Fragile X syndrome, the most common cause of inherited mental retardation, is caused by the absence of FMRP (Fragile X Mental Retardation Protein). FMRP is an RNA binding protein reported to be involved in translational control, notably at postsynaptic sites of protein synthesis as a part of a multiprotein/mRNA complex[PMID:12941608]. NUFIP1 is one of the several FMRP-interacting proteins. NUFIP can act as a pol II-specific basal transcriptional activator in vitro and when ectopically overexpressed in vivo. NUFIP can directly activate promoters by enhancing the ATP-dependent release of hyperphosphorylated form of pol II from open transcription complexes[PMID:15107825].