Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17752-1-AP targets NUFIP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUFIP2 fusion protein Ag12094 相同性解析による交差性が予測される生物種 |
| Full Name | nuclear fragile X mental retardation protein interacting protein 2 |
| Calculated molecular weight | 695 aa, 76 kDa |
| Observed molecular weight | 76-82 kDa |
| GenBank accession number | BC108307 |
| Gene Symbol | NUFIP2 |
| Gene ID (NCBI) | 57532 |
| RRID | AB_2878433 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z417 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IHC protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IF protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| IP protocol for NUFIP2 antibody 17752-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Proc Natl Acad Sci U S A Succinyl-CoA ligase ADP-forming subunit beta promotes stress granule assembly to regulate redox and drive cancer metastasis | ||
J Cell Biol Proteomic and 3D structure analyses highlight the C/D box snoRNP assembly mechanism and its control. | ||
Neuron Increased G3BP2-Tau interaction in tauopathies is a natural defense against Tau aggregation | ||
Cell Rep The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network |