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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUFIP2 Monoclonal antibody, PBS Only

NUFIP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4E3

Cat no : 67195-1-PBS

Synonyms

Cell proliferation-inducing gene 1 protein, 82-FIP, 82 kDa FMRP-interacting protein, 82 FIP, 2A4E3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUFIP2 Monoclonal antibody (67195-1-Ig)

    WB analysis using 67195-1-Ig (same clone as 67195-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HSC-T6 cells using NUFIP2 Monoclonal antibody (67195-1-Ig)

    WB analysis of HSC-T6 using 67195-1-Ig (same clone as 67195-1-PBS)

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using NUFIP2 Monoclonal antibody (67195-1-Ig)

    WB analysis of NIH/3T3 using 67195-1-Ig (same clone as 67195-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of 4T1 cells using NUFIP2 Monoclonal antibody (67195-1-Ig)

    WB analysis of 4T1 using 67195-1-Ig (same clone as 67195-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 67195-1-Ig (NUFIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUFIP2 Monoclonal antibody (67195-1-Ig)

    IHC staining of mouse brain using 67195-1-Ig (same clone as 67195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NUFIP2 Monoclonal antibody (67195-1-Ig)

    IHC staining of mouse cerebellum using 67195-1-Ig (same clone as 67195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse cerebellum tissue using NUFIP2 Monoclonal antibody (67195-1-Ig)

    IHC staining of mouse cerebellum using 67195-1-Ig (same clone as 67195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUFIP2 Monoclonal antibody (67195-1-Ig)

    IHC staining of mouse brain using 67195-1-Ig (same clone as 67195-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67195-1-Ig (NUFIP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NUFIP2 Monoclonal antibody (67195-1-Ig)

    IF Staining of HepG2 using 67195-1-Ig (same clone as 67195-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using NUFIP2 antibody (67195-1-Ig, Clone: 2A4E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67195-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67195-1-PBS targets NUFIP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen NUFIP2 fusion protein Ag29040 相同性解析による交差性が予測される生物種
Full Name nuclear fragile X mental retardation protein interacting protein 2
Calculated molecular weight 695 aa, 76 kDa
Observed molecular weight 76 kDa, 82 kDa
GenBank accession numberBC108307
Gene symbol NUFIP2
Gene ID (NCBI) 57532
RRIDAB_2882489
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.