Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICC

WB analysis of mouse brain using 10111-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody at dilution of 1:2000 incubated at 4 degree celsius over night.
WB analysis of HeLa using 10111-1-AP
WB result of NUMBL antibody (10111-1-AP, 1:3000) with si-Control and si-NUMBL transfected HeLa cells.
WB analysis of HeLa using 10111-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse spleen using 10111-1-AP
mouse spleen tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 10111-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10111-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10111-1-AP (NUMBL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 10111-1-AP
IP result of anti-NUMBL (IP:10111-1-AP, 4ug; Detection:10111-1-AP 1:1000) with HeLa cells lysate 2800 ug.
IHC staining of human brain using 10111-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 10111-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 10111-1-AP (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 10111-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 10111-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10111-1-AP (NUMBL antibody at dilution of 1:50 (under 40x lens).
IF Staining of mouse cerebellum using 10111-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using NUMBL antibody (10111-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of PC-12 using 10111-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 10111-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10111-1-AP (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

NUMBL Polyclonal antibody

NUMBL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 10111-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

Numb-related protein, Numb-like protein, NUMBLIKE

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human brain tissue, human gliomas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse cerebellum tissue
Positive IF/ICC detected in	PC-12 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IHC	See 3 publications below

Product Information

10111-1-AP targets NUMBL in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NUMBL fusion protein Ag0156 相同性解析による交差性が予測される生物種
Full Name	numb homolog (Drosophila)-like
Calculated molecular weight	65 kDa
Observed molecular weight	65-70 kDa
GenBank accession number	BC001794
Gene Symbol	NUMBL
Gene ID (NCBI)	9253
RRID	AB_2154440
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y6R0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.

Protocols

Product Specific Protocols
WB protocol for NUMBL antibody 10111-1-AP	Download protocol
IHC protocol for NUMBL antibody 10111-1-AP	Download protocol
IF protocol for NUMBL antibody 10111-1-AP	Download protocol
IP protocol for NUMBL antibody 10111-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Neurosci Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. Authors - Rasin Mladen-Roko MR View Article
human	WB,IHC	Cancer Res Regulation of lung cancer metastasis by Klf4-Numb-like signaling. Authors - Vaira Valentina V KD Validated View Article
human	WB,IHC	J Clin Endocrinol Metab PMID: 16705073 Authors - Sinha-Hikim Indrani I View Article
mouse	WB	Development Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways. Authors - Yusuke Okubo View Article
mouse	IHC	Mol Endocrinol Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation. Authors - Moran Tyler B TB View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse brain using 10111-1-AP

WB analysis of HeLa using 10111-1-AP

WB analysis of HeLa using 10111-1-AP

WB analysis of mouse spleen using 10111-1-AP

WB analysis of mouse lung using 10111-1-AP

WB analysis of mouse liver using 10111-1-AP

IP experiment of HeLa using 10111-1-AP

IHC staining of human brain using 10111-1-AP

IHC staining of human brain using 10111-1-AP

IHC staining of human gliomas using 10111-1-AP

IHC staining of human gliomas using 10111-1-AP

IF Staining of mouse cerebellum using 10111-1-AP

IF Staining of PC-12 using 10111-1-AP

IF Staining of HepG2 using 10111-1-AP

NUMBL Polyclonal antibody

NUMBL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors.

Regulation of lung cancer metastasis by Klf4-Numb-like signaling.

PMID: 16705073

Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways.

Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation.