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- KD/KO Validated
NUMBL Polyclonal antibody
NUMBL Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 10111-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, mouse spleen tissue, HeLa cells, mouse lung tissue, mouse liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human brain tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse cerebellum tissue |
Positive IF/ICC detected in | PC-12 cells, HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 3 publications below |
Product Information
10111-1-AP targets NUMBL in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | NUMBL fusion protein Ag0156 相同性解析による交差性が予測される生物種 |
Full Name | numb homolog (Drosophila)-like |
Calculated molecular weight | 65 kDa |
Observed molecular weight | 65-70 kDa |
GenBank accession number | BC001794 |
Gene symbol | NUMBL |
Gene ID (NCBI) | 9253 |
RRID | AB_2154440 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specifically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for NUMBL antibody 10111-1-AP | Download protocol |
IHC protocol for NUMBL antibody 10111-1-AP | Download protocol |
IF protocol for NUMBL antibody 10111-1-AP | Download protocol |
IP protocol for NUMBL antibody 10111-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Neurosci Numb and Numbl are required for maintenance of cadherin-based adhesion and polarity of neural progenitors. | ||
Development Cleaved Delta like 1 intracellular domain regulates neural development via Notch signal-dependent and -independent pathways. | ||
Mol Endocrinol Numb deletion in POMC-expressing cells impairs pituitary intermediate lobe cell adhesion, progenitor cell localization, and neuro-intermediate lobe boundary formation. |