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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using NUMBL Monoclonal antibody (66155-1-Ig)

    WB analysis of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 66155-1-Ig (NUMBL antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUMBL Monoclonal antibody (66155-1-Ig)

    IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using NUMBL Monoclonal antibody (66155-1-Ig)

    IHC staining of mouse brain using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66155-1-Ig (NUMBL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NUMBL Monoclonal antibody (66155-1-Ig)

    IHC staining of human gliomas using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using NUMBL Monoclonal antibody (66155-1-Ig)

    IHC staining of human gliomas using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66155-1-Ig (NUMBL antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using NUMBL Monoclonal antibody (66155-1-Ig)

    IF Staining of PC-12 using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed PC-12 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using NUMBL Monoclonal antibody (66155-1-Ig)

    IF Staining of L02 using 66155-1-Ig (same clone as 66155-1-PBS)

    Immunofluorescent analysis of L02 cells using 66155-1-Ig (NUMBL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66155-1-PBS in a different storage buffer formulation.

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NUMBL Monoclonal antibody, PBS Only

NUMBL Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66155-1-PBS
Clone No.5F6E1

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

CAG3A, CTG3a, NBL, numb homolog (Drosophila) like, Numb like protein, NUMB R, Numb related protein, NUMBL, NUMBLIKE, NUMBR, TNRC23

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66155-1-PBS targets NUMBL in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen NUMBL fusion protein Ag16771 相同性解析による交差性が予測される生物種
Full Name numb homolog (Drosophila)-like
Calculated molecular weight 65 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC001794
Gene Symbol NUMBL
Gene ID (NCBI) 9253
RRIDAB_2881551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6R0
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Numbl (also known as numblike or Nbl), as a conserved homolog of Drosophila Numb, is specfically expressed in the brain. Numbl is a cytoplasm protein and has redundant functions in embryonic neurogenesis. It plays an important role in ependymal wall integrity and subventricular zone neuroblast survival. However, its expression and function in the central nervous system lesion are still unclear.