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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP153 Polyclonal antibody, PBS Only

NUP153 Polyclonal Antibody for WB, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14189-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis using 14189-1-AP (same clone as 14189-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using NUP153 Polyclonal antibody (14189-1-AP)

    WB analysis of A431 using 14189-1-AP (same clone as 14189-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 14189-1-AP (NUP153 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using NUP153 Polyclonal antibody (14189-1-AP)

    IP experiment of K-562 using 14189-1-AP (same clone as 14189-1-PBS)

    IP result of anti-NUP153 (IP:14189-1-AP, 4ug; Detection:14189-1-AP 1:500) with K-562 cells lysate 3600ug. This data was developed using the same antibody clone with 14189-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14189-1-PBS targets NUP153 in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP153 fusion protein Ag5519 相同性解析による交差性が予測される生物種
Full Name nucleoporin 153kDa
Calculated molecular weight 154 kDa
Observed molecular weight 154 kDa
GenBank accession numberBC052965
Gene symbol NUP153
Gene ID (NCBI) 9972
RRIDAB_2154463
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

NUP153 is an FG-repeat containing NUP, contains a high-affinity site for importin-β, localizes on the nucleoplasmic face of the nuclear pore and is required for assembly of the nuclear basket and import of a subset of nuclear proteins [PMID:18845677]. NUP153 also facilitates nuclear export of mRNA and ribonucleoprotein particles and may have additional functions within the nucleus [PMID:16195343]. The 53BP1-NUP153/importin-β pathway as an important aspect of the DDR network add to an emerging evidence of subcellular trafficking as an integral part of genome surveillance [PMID:22075984].