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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP53 Polyclonal antibody

NUP53 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 19819-1-AP

Synonyms

35 kDa nucleoporin, Mitotic phosphoprotein 44, MP 44, MP44, nucleoporin 35kDa, Nucleoporin Nup35, Nucleoporin NUP53, NUP35, NUP53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP53 Polyclonal antibody (19819-1-AP)

    WB analysis using 19819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NUP53 Polyclonal antibody (19819-1-AP)

    WB analysis of K-562 using 19819-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 19819-1-AP (NUP53 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, K-562 cells, HeLa cells, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 19819-1-AP is NUP53 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP53 fusion protein Ag13869 相同性解析による交差性が予測される生物種
Full Name nucleoporin 35kDa
Calculated molecular weight 35 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC061698
Gene symbol NUP53
Gene ID (NCBI) 129401
RRIDAB_2878611
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUP53 antibody 19819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols