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NUP50 Monoclonal antibody

NUP50 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D5A7

Cat no : 67001-1-Ig

Synonyms

50 kDa nucleoporin, nucleoporin 50kDa, Nucleoporin Nup50, NUP50

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using NUP50 Monoclonal antibody (67001-1-Ig)

    WB analysis using 67001-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of HEK-293 cells using NUP50 Monoclonal antibody (67001-1-Ig)

    WB analysis of HEK-293 using 67001-1-Ig

    WB result of NUP50 antibody (67001-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NUP50 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using NUP50 Monoclonal antibody (67001-1-Ig)

    WB analysis using 67001-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using NUP50 Monoclonal antibody (67001-1-Ig)

    WB analysis using 67001-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67001-1-Ig (NUP50 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP50 Monoclonal antibody (67001-1-Ig)

    IHC staining of human breast cancer using 67001-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NUP50 Monoclonal antibody (67001-1-Ig)

    IHC staining of human breast cancer using 67001-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67001-1-Ig (NUP50 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, HEK-293 cells, Ramos cells, Daudi cels, Sp2/0 cells, MCF-7 cells, HT-29 cells, U2OS cells, HepG2 cells, Jurkat cells, MOLT-4 cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67001-1-Ig targets NUP50 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NUP50 fusion protein Ag15144 相同性解析による交差性が予測される生物種
Full Name nucleoporin 50kDa
Calculated molecular weight 468 aa, 50 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC028125
Gene symbol NUP50
Gene ID (NCBI) 10762
RRIDAB_2882318
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nup50 (also known as Npap60) is a nucleoporin that binds directly to importin α and their interaction plays an important role in nuclear import. Nup50 is a mobile nucleoporin localized primarily in the nuclear basket of the nuclear pore complex (NPC) and in the nucleoplasm. Human Nup50 has two isoforms: Npap60L (1-469 aa), and its alternatively spliced isoform, Npap60S (29-469 aa) of Npap60L.

Protocols

Product Specific Protocols
WB protocol for NUP50 antibody 67001-1-IgDownload protocol
IHC protocol for NUP50 antibody 67001-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols