- Featured Product
- KD/KO Validated
NUP62 Polyclonal antibody
NUP62 Polyclonal Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, IP, ELISA
Conjugate
Unconjugated
Cat no : 13916-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 13 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
13916-1-AP targets NUP62 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NUP62 fusion protein Ag4912 相同性解析による交差性が予測される生物種 |
| Full Name | nucleoporin 62kDa |
| Calculated molecular weight | 522 aa, 53 kDa |
| Observed molecular weight | 62 kDa |
| GenBank accession number | BC014842 |
| Gene symbol | NUP62 |
| Gene ID (NCBI) | 23636 |
| RRID | AB_2267660 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
NUP62 belongs to the nucleoporin NSP1/NUP62 family. It is essential component of the nuclear pore complex. The N-terminal is probably involved in nucleocytoplasmic transport. The C-terminal is probably involved in protein-protein interaction via coiled-coil formation and may function in anchorage of p62 to the pore complex. Defects in NUP62 are the cause of infantile striatonigral degeneration (SNDI). NUP62 consists of a single promoter with a CpG island and three transcribed exons. It is ubiquitously expressed, an observation compatible with its essential role in transporting cargo across the nuclear envelope. (PMID:16029492)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NUP62 antibody 13916-1-AP | Download protocol |
| IHC protocol for NUP62 antibody 13916-1-AP | Download protocol |
| IP protocol for NUP62 antibody 13916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Microbiol Nuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus. | ||
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
PLoS Genet Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells. | ||
iScience Tat-P combined with GAPR1 releases Beclin1 to promote autophagy and improve Bronchopulmonary dysplasia model | ||
