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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

NUP62CL Polyclonal antibody

NUP62CL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25381-1-AP

Synonyms

NUP62CL, NUP62L

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of HeLa using 25381-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of Jurkat using 25381-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using NUP62CL Polyclonal antibody (25381-1-AP)

    WB analysis of SH-SY5Y using 25381-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25381-1-AP (NUP62CL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using NUP62CL Polyclonal antibody (25381-1-AP)

    IHC staining of human lung cancer using 25381-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 25381-1-AP (NUP62CL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, SH-SY5Y cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25381-1-AP targets NUP62CL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NUP62CL fusion protein Ag21107 相同性解析による交差性が予測される生物種
Full Name nucleoporin 62kDa C-terminal like
Calculated molecular weight 72 aa, 8 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC017799
Gene symbol NUP62CL
Gene ID (NCBI) 54830
RRIDAB_2880051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for NUP62CL antibody 25381-1-APDownload protocol
IHC protocol for NUP62CL antibody 25381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols